Brittany Xavier here's two reasons why we don't personally use Tylenol or acetaminophen Instagram Frontiers Multiscale zonation resolved modeling of dose dependent determinants of acetaminophen induced liver injury Interleukin 4 deficiency protects mice from acetaminophen induced liver injury and inflammation by prevention of glutathione depletion Inflammation Research Springer Nature Link Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione, Tylenol & Your Health: What You Should Know